![]() Iterons (DR) are present and are bound by RepE molecules, whichĬould cause local DNA melting at the 13-mer region with the assistance of a histone-like protein HU and subsequent initiation of Within the replication origin (ori2), four directly repeated Sequence DNA (iteron Masson & Ray, 1986, 1988 Tokino et al.,ġ986). Monomer is a replication initiator that recognizes a specific 19 bp States, monomeric and dimeric, of RepE have been found and theirįunctions are known to be different (Ish iai et al., 1994). Role in the regulation of mini-F replication. Initiator protein RepE (251 amino acids, 29 kDa) plays an important Of 1–2 per host chromosome (Kline, 1985). The mini-F plasmid, which is a derivative of the F factor in Escherichia coli, replicates under strict control, maintaining a copy number ![]() ![]() # 2007 International Union of Crystallography The application of dehydration and crystal annealing and belong to space group The crystals diffracted to a resolution of 3.14 Å after Protein, purified under native conditions with a high salt concentration andĬrystallized in complex with the repE operator DNA using the sitting-drop Wild-type dimeric RepE protein was expressed as an N-terminal histidine-tagged Initiator, while its dimer acts as a transcriptional repressor of the repE gene. RepE protein has a dual function: its monomer functions as a replication The replication initiator factor RepE of the F plasmid in Escherichia coli is anĮssential protein that stringently regulates the F-plasmid copy number. Kyoto 606-8315, Japan, and cRIKEN SPring-8Ĭenter at Harima Institute, Koto 1-1-1, Sayo, Kyoto 606-8502, Japan, bGraduate School of Preliminary X-ray crystallographic study of itsĭepartment of Chemistry, Graduate School of Expression and purification of F-plasmid RepE and
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